STAR Protocols and manuals
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All the protocols used during experiments conducted in STAR are available here.
Two protocols are described in detail, these are:
- Endoreduplication is a typical response of plants to avoid damage by different abiotic stressors; this is estimated by
measuring the ploidy of plants in isolated nuclei (SCK•CEN)
Exposure of the nematode worm Caenorhabditis elegans to radionuclides during its whole life cycle for the study of toxic effects on growth and reproduction kinetics (IRSN)
Summarised information is available for the following:
Arabidopsis thaliana Culture in Hydroponics
Area and frond estimation in Image J
cDNA synthesis for use in RT-PCR
Checking RNA-content of samples with the NANODROP
Checking RNA quality with the BIOANALYZER using the RNA 6000 nano kit
Continuous culture of Lemna minor
Enzyme Analysis by Spectrophotometry - Plate Reader method
Harvest of lemna minor
Lipid peroxidation TBA/TCA plate reader method
Metabolites measurements with the plate reader
Mineralisation of plant material for determination of metal content
OECD guidelines for the testing of chemicals
Photosynthesis - examples of results
Photosynthesis measurement in Arabidopsis thalliana using the DUAL-PAM
RNA extraction from plant samples
Two-step RT-PCR analysis